Mannich bases derivatives of 2-Phenyl-5-Benzimidazole sulfonic acid; Synthesis, Characterization, Computational studies and Biological evaluation

A new series of N-Mannich bases 2-Phenyl-5-benzimidazole sulfonic acid have been synthesized through amino methylation reaction with secondary amines. The two moieties were held together a methylene bridge, which comes from formaldehyde (Formalin Solution 37%) used in the reaction. Chemical structures newly compounds confirmed using FT-IR, 1HNMR and 13CNMR. Different vitro assays including Anti-oxidant, Enzyme inhibition, Anti-microbial Cytotoxicity assay performed to evaluate biological potential reference standard drug. Among library, compound 3a shows maximum alpha-glucosidase inhibition an IC50 value 66.66 μg/ml, 3d was found most toxic LC50 10.17 μg/ml. ADME evaluation studies help Molinspiration online software. Docking calculations also performed. Given importance nucleus involved, might find extensive medicinal applications as reported literature.